Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse brain tissue, mouse pancreas tissue, rat heart tissue, human pancreas tissue, rat pancreas tissue, human stomach cancer tissue, mouse heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66625-1-Ig targets RIC8A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1593 Product name: Recombinant human RIC8A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 117-467 aa of BC011821 Sequence: QQLFQELKGVRLLTDTLELTLGVTPEGNPPTLLPSQETERAMEILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD Predict reactive species |
| Full Name | resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC011821 |
| Gene Symbol | RIC8A |
| Gene ID (NCBI) | 60626 |
| RRID | AB_2881985 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NPQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| IP protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| WB protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| IF protocol for RIC8A antibody 66625-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIC8A Monoclonal antibody (1E3B11), catalog number 66625-1-Ig, has 2 total citations. These appear in Journal of Clinical Investigation and Journal of Biological Chemistry. The cited research focuses on G-protein signaling, specifically involving neomorphic Gαo mutations in GNAO1 encephalopathies and the regulation of constitutively active Gαq by the guanine nucleotide exchange factor Ric-8A. Both studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
J Clin Invest Neomorphic Gαo mutations gain interaction with Ric8 proteins in GNAO1 encephalopathies | ||
J Biol Chem The guanine nucleotide exchange factor Ric-8A regulates the sensitivity of constitutively active Gαq to the inhibitor YM-254890
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