Tested Applications
| Positive WB detected in | Raji cells, THP-1 cells, Ramos cells, Daudi cells |
| Positive IP detected in | MDA-MB-231 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66721-1-Ig targets SYK in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17654 Product name: Recombinant human SYK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 239-427 aa of BC001645 Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL Predict reactive species |
| Full Name | spleen tyrosine kinase |
| Calculated Molecular Weight | 635 aa, 72 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC001645 |
| Gene Symbol | SYK |
| Gene ID (NCBI) | 6850 |
| ENSEMBL Gene ID | ENSG00000165025 |
| RRID | AB_2882072 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SYK antibody 66721-1-Ig | Download protocol |
| IHC protocol for SYK antibody 66721-1-Ig | Download protocol |
| WB protocol for SYK antibody 66721-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SYK Monoclonal antibody (catalog number 66721-1-Ig) has 9 total citations. These appear in journals including Cancer Cell, Cancer Letters, Cell Reports, Cell Biology and Toxicology, PLoS Pathogens, Experimental Cell Research, Gene, and F1000Research. The associated research fields span oncology (glioblastoma, pancreatic cancer, hepatocellular carcinoma, acute myeloid leukemia), immunology and inflammation (lupus nephritis, inflammasome activation, diabetic retinopathy), and antibody validation for Western blot, immunoprecipitation, and immunofluorescence applications.
| Species | Application | Title |
|---|---|---|
Cancer Cell Infiltrating plasma cells maintain glioblastoma stem cells through IgG-Tumor binding | ||
Cancer Cell Distinct roles of TREM2 in central nervous system cancers and peripheral cancers | ||
Cancer Lett SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer. | ||
Cell Rep Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses | ||
Cell Biol Toxicol Troxerutin suppresses the stemness of hepatocellular carcinoma via the Syk/FOXO3 feedback loop. | ||
PLoS Pathog Pseudorabies Virus UL4 protein promotes the ASC-dependent inflammasome activation and pyroptosis to exacerbate inflammation |
