Tested Applications
| Positive WB detected in | HeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:100-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66775-1-Ig targets Syntaxin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27926 Product name: Recombinant human STX16 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-304 aa of BC019042 Sequence: MATRRLTDAFLLLRNNSIQNRQLLAEQLADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGRIKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHRCQRAVQALPSRARACSEQEGRLLGNVVASLAQALQELSTSFRHAQSGYLKRMKNREERSQHFFDTSVPLMDDGDDNTLYHRGFTEDQLVLVEQNTLMVEEREREIRQIVQSISDLNEIFRDLGAMIVEQGTVLDRIDYNVEQSCIKTEDGLKQLHKAEQYQKKNRKMLVILILFVIIIVLIVVLVGVKSR Predict reactive species |
| Full Name | syntaxin 16 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC019042 |
| Gene Symbol | Syntaxin 16 |
| Gene ID (NCBI) | 8675 |
| RRID | AB_2882121 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14662 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Syntaxin 16 antibody 66775-1-Ig | Download protocol |
| IHC protocol for Syntaxin 16 antibody 66775-1-Ig | Download protocol |
| WB protocol for Syntaxin 16 antibody 66775-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Syntaxin 16 Monoclonal antibody (4A9D8), catalog number 66775-1-Ig, has 2 total citations. These appear in Cell Mol Life Sci (2025) and J Gastroenterol (2025). The cited research spans cardiovascular/cell biology (hypoxic adipose stem cell exosomes alleviating ischemia-reperfusion injury) and oncology (hepatocellular carcinoma progression via β1 integrin recycling). Both citations used the antibody for Western blot in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci VAMP4 in hypoxic adipose stem cell exosomes alleviates ischemia-reperfusion injury. | ||
J Gastroenterol VPS45 promotes the progression of hepatocellular carcinoma by recycling β1 integrin to the cell membrane via the endocytic pathway. |
