Filter: AllWBSample testIHCIF/ICCCytometric bead array

WB analysis using 66775-1-Ig (same clone as 66775-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66775-1-Ig (same clone as 66775-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 66775-1-Ig (same clone as 66775-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
Sample test of MP51331-1
Sample test of MP51331-1, STX16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66775-1-PBS. Detection antibody: 66775-2-PBS.
IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66775-1-Ig (same clone as 66775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66775-1-Ig (same clone as 66775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 66775-1-Ig (same clone as 66775-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 66775-1-Ig (same clone as 66775-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66775-1-Ig (Syntaxin 16 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
IF Staining of Neuro-2a using 66775-1-Ig (same clone as 66775-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using Syntaxin 16 antibody (66775-1-Ig, Clone: 4A9D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66775-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51331-1
Cytometric bead array standard curve of MP51331-1, STX16 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66775-1-PBS. Detection antibody: 66775-2-PBS. Standard:Ag27926. Range: 1.563-200 ng/mL.

Syntaxin 16 Monoclonal antibody, PBS Only (Capture)

Syntaxin 16 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66775-1-PBS

Clone No.4A9D8

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

hsyn16, STX16, SYN16, syntaxin 16

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66775-1-PBS targets Syntaxin 16 as part of a matched antibody pair:

MP51331-1: 66775-1-PBS capture and 66775-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Syntaxin 16 fusion protein Ag27926 Predict reactive species
Full Name	syntaxin 16
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	37-40 kDa
GenBank Accession Number	BC019042
Gene Symbol	Syntaxin 16
Gene ID (NCBI)	8675
RRID	AB_2882121
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O14662
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.