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  • Western Blot (WB) analysis of A431 cells using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    WB analysis of A431 using 66906-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin alpha-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of JAR cells using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    WB analysis of JAR using 66906-1-Ig

    JAR cells were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin Alpha 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Integrin Alpha 6 Monoclonal antibody (66906-1-Ig)

    WB analysis of human placenta using 66906-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin Alpha 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Integrin alpha 6 Monoclonal antibody

Integrin alpha 6 Monoclonal Antibody for WB, ELISA

Cat No. 66906-1-Ig
Clone No.2B12A1

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

ITGA6, 2B12A1, CD49 antigen-like family member F, CD49f, Integrin alpha-6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA431 cells, JAR cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66906-1-Ig targets Integrin alpha 6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Integrin alpha 6 fusion protein Ag25866 Predict reactive species
Full Name integrin, alpha 6
Calculated Molecular Weight 1073 aa, 119 kDa
Observed Molecular Weight 115-120 kDa
GenBank Accession NumberBC136456
Gene Symbol Integrin alpha 6
Gene ID (NCBI) 3655
RRIDAB_2882233
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23229
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).

Protocols

Product Specific Protocols
WB protocol for Integrin alpha 6 antibody 66906-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols