Tested Applications
| Positive WB detected in | A431 cells, JAR cells, human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66906-1-Ig targets Integrin alpha 6 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25866 Product name: Recombinant human Integrin alpha-6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 477-690 aa of BC136456 Sequence: TPNRIDLRQKTACGAPSGICLQVKSCFEYTANPAGYNPSISIVGTLEAEKERRKSGLSSRVQFRNQGSEPKYTQELTLKRQKQKVCMEETLWLQDNIRDKLRPIPITASVEIQEPSSRRRVNSLPEVLPILNSDEPKTAHIDVHFLKEGCGDDNVCNSNLKLEYKFCTREGNQDKFSYLPIQKGVPELVLKDQKDIALEITVTNSPSNPRNPTK Predict reactive species |
| Full Name | integrin, alpha 6 |
| Calculated Molecular Weight | 1073 aa, 119 kDa |
| Observed Molecular Weight | 115-120 kDa |
| GenBank Accession Number | BC136456 |
| Gene Symbol | Integrin alpha 6 |
| Gene ID (NCBI) | 3655 |
| RRID | AB_2882233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23229 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin alpha 6 antibody 66906-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Integrin alpha 6 Monoclonal antibody (2B12A1), catalog number 66906-1-Ig, has a total of 3 citations. These appear in Nature Communications, Biochemical and Biophysical Research Communications, and British Journal of Pharmacology. The associated research fields include tissue engineering and synthetic cell integration, hair follicle stem cell biology and NFATc1 signaling, and cardiac pharmacology involving integrin-mediated hypertrophy inhibition.
| Species | Application | Title |
|---|---|---|
Nat Commun Self-assembly of hybrid 3D cultures by integrating living and synthetic cells. | ||
Biochem Biophys Res Commun MafB regulates hair follicle stem cell activation and hair regeneration through NFATc1 signaling pathway | ||
Br J Pharmacol Empagliflozin inhibits cardiac hypertrophy via a mechanism involving integrin signalling. |
