TBL1X Monoclonal antibody

TBL1X Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66955-1-Ig

Clone No.1A9A1

Publications(2)

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EBI, SMAP55, TBL1, TBL1X

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

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Filter: AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 66955-1-Ig
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66955-1-Ig (TBL1X antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 66955-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 66955-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 66955-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 66955-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66955-1-Ig (TBL1X antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 66955-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TBL1X antibody (66955-1-Ig, Clone: 1A9A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:100000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66955-1-Ig targets TBL1X in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity	Human, Mouse, Rat
Cited Reactivity	human, mouse
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5291 Product name: Recombinant human TBL1X protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-362 aa of BC032708 Sequence: MSITSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGTLVPPAALISILQKGLQYVEAEISINEDGTVFDGRPIESLSLIDAVMPDVVQTRQQAFREKLAQQQASAAAAAAAATAAATAATTTSAGVSHQNPSKNREATVNGEENRAHSVNNHAKPMEIDGEVEIPSSKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNTNGTLLATGSYDGFARIWTEDGNLASTLGQHKGPIFALKWNRKGNYILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTFASCSTDMCIHVCRLGCDRPVKTFQGHTN Predict reactive species
Full Name	transducin (beta)-like 1X-linked
Calculated Molecular Weight	526 aa, 57 kDa
Observed Molecular Weight	57 kDa, 62 kDa
GenBank Accession Number	BC032708
Gene Symbol	TBL1X
Gene ID (NCBI)	6907
RRID	AB_2882278
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O60907
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for TBL1X antibody 66955-1-Ig	Download protocol
IHC protocol for TBL1X antibody 66955-1-Ig	Download protocol
WB protocol for TBL1X antibody 66955-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse,human	WB	Arterioscler Thromb Vasc Biol Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice Authors - Mei Yang View Article
human	WB	J Virol Interaction of NEP with G Protein Pathway Suppressor 2 Facilitates Influenza A Virus Replication by Weakening the Inhibition of GPS2 to RNA Synthesis and Ribonucleoprotein Assembly. Authors - Wenxiao Gong View Article