Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, LNCaP cells, HEK-293 cells, rat brain tissue, mouse brain tissue, HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue, human stomach cancer tissue, human colon cancer tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66977-1-Ig targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28427 Product name: Recombinant human CAPN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-330 aa of BC021303 Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD Predict reactive species |
| Full Name | calpain 2, (m/II) large subunit |
| Calculated Molecular Weight | 700 aa, 80 kDa |
| Observed Molecular Weight | 72-80 kDa |
| GenBank Accession Number | BC021303 |
| Gene Symbol | CAPN2 |
| Gene ID (NCBI) | 824 |
| RRID | AB_2882297 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P17655 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928, and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from the human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAPN2 antibody 66977-1-Ig | Download protocol |
| IHC protocol for CAPN2 antibody 66977-1-Ig | Download protocol |
| WB protocol for CAPN2 antibody 66977-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAPN2 Monoclonal antibody (1E1F10), catalog number 66977-1-Ig, has 5 total citations published in Advanced Science, International Journal of Surgery, Oxidative Medicine and Cell Longevity, Cell Molecular Life Sciences, and EMBO Reports. Associated research fields include rheumatoid arthritis and fibroblast biology, pancreatic cancer metastasis, depression and oxidative stress signaling, spinocerebellar ataxia pathogenesis, and NMDAR-dependent local translation in neuroscience.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Inhibition of Aberrant Activated Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Leishmania Peptide via the Regulation of Fatty Acid Synthesis Metabolism | ||
Int J Surg METTL16 inhibits pancreatic cancer proliferation and metastasis by promoting MROH8 RNA stability and inhibiting CAPN2 expression | ||
Oxid Med Cell Longev Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis. | ||
Cell Mol Life Sci Calpains as novel players in the molecular pathogenesis of spinocerebellar ataxia type 17. | ||
EMBO Rep Proteolytic cleavage of G3BP1 by calpain 1 couples NMDAR activation to mTOR-dependent local translation.
|
