CAPN2 Monoclonal antibody, PBS Only

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  WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  WB analysis using 66977-1-Ig (same clone as 66977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66977-1-Ig (same clone as 66977-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66977-1-Ig (same clone as 66977-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66977-1-Ig (same clone as 66977-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66977-1-Ig (CAPN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66977-1-Ig (same clone as 66977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66977-1-Ig (same clone as 66977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66977-1-Ig (same clone as 66977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66977-1-Ig (same clone as 66977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66977-1-Ig (CAPN2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66977-1-Ig (same clone as 66977-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CAPN2 antibody (66977-1-Ig, Clone: 1E1F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66977-1-PBS in a different storage buffer formulation.

CAPN2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66977-1-PBS

Clone No.1E1F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1E1F10, Calcium-activated neutral proteinase 2, Calpain 2, Calpain 2 catalytic subunit, Calpain 2 large subunit

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Product Information

66977-1-PBS targets CAPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28427

  Product name: Recombinant human CAPN2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-330 aa of BC021303

  Sequence: MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSD

• Full Name: calpain 2, (m/II) large subunit
• Calculated Molecular Weight: 700 aa, 80 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC021303
• Gene Symbol: CAPN2
• Gene ID (NCBI): 824
• RRID: AB_2882297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P17655
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.