Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, T-47D cells, PC-3 cells, HepG2 cells, HSC-T6 cells |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66996-1-Ig targets CEP63 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9227 Product name: Recombinant human CEP63 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-349 aa of BC014050 Sequence: MEALLEGIQNRGHGGGFLTSCEAELQELMKQIDIMVAHKKSEWEGRTHALETCLKIREQELKSLRSQLDVTHKEVGMLHQQVEEHEKIKQEMTMEYKQELKKLHEELCILKRSYEKLQKKQMREFRGNTKNHREDRSEIERLTAKIEEFRQKSLDWEKQRLIYQQQVSSLEAQRKALAEQSEIIQAQLVNRKQKLESVELSSQSEIQHLSSKLERANDTICANELEIERLTMRVNDLVGTSMTVLQEQQQKEEKLRESEKLLEALQEEKRELKAALQSQENLIHEARIQKEKLQEKVKATNTQHAVEAISLESVSATCKQLSQELMEKYEELKRMEAHNNEYKAEIKKL Predict reactive species |
| Full Name | centrosomal protein 63kDa |
| Calculated Molecular Weight | 703 aa, 81 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC014050 |
| Gene Symbol | CEP63 |
| Gene ID (NCBI) | 80254 |
| RRID | AB_2882313 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96MT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP63 is required for normal spindle assembly. It maintains centrosome numbers through centrosomal recruitment of CEP152. CEP63 recruits CDK1 to centrosomes. It plays a role in DNA damage response.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP63 antibody 66996-1-Ig | Download protocol |
| WB protocol for CEP63 antibody 66996-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CEP63 Monoclonal antibody (2G12B2), catalog number 66996-1-Ig, has 2 total citations. These appear in Nature Communications and Neural Regen Res. The cited research focuses on centriole biogenesis involving the intrinsically disordered protein ALMS1, and on CNKSR2 interactome analysis related to the centrosome/microtubule system. The application used in the cited studies was immunofluorescence (IF) in mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Adaptable centriole biogenesis via the intrinsically disordered protein ALMS1. | ||
Neural Regen Res CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system |
