Tested Applications
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HT-29 cells, Caco-2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67052-1-Ig targets SMAD5 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28699 Product name: Recombinant human SMAD5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 161-465 aa of BC009682 Sequence: SHNEPHMPQNATFPDSFHQPNNTPFPLSPNSPYPPSPASSTYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKSRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNFHHGFHPTTVCKIPSSCSLKIFNNQEFAQLLAQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPLNPISSVS Predict reactive species |
| Full Name | SMAD family member 5 |
| Calculated Molecular Weight | 465 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC009682 |
| Gene Symbol | SMAD5 |
| Gene ID (NCBI) | 4090 |
| RRID | AB_2882365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99717 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMAD5 antibody 67052-1-Ig | Download protocol |
| WB protocol for SMAD5 antibody 67052-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SMAD5 Monoclonal antibody (1A6A4), catalog number 67052-1-Ig, has 9 citations. These appear in journals including Cancer Letters, Acta Biomaterialia, Frontiers in Cell and Developmental Biology, Journal of Cellular Physiology, Bone, Molecular and Cellular Endocrinology, FEBS Letters, Journal of Cellular Biochemistry, and Applied Bionics and Biomechanics. Research fields span colorectal cancer, osteoporosis and bone biology, gastric cancer, BMP/SMAD signaling, endothelial protection, and angiogenesis.
| Species | Application | Title |
|---|---|---|
Cancer Lett Cancer Associated Fibroblasts-derived Lactate Induces Oxaliplatin Treatment Resistance by Promoting Cancer Stemness via ANTXR1 Lactylation in Colorectal Cancer | ||
Acta Biomater Acid-responsive bone-targeting nanoplatform enables spatiotemporal control of osteogenesis and angiogenesis for osteoporosis rescue. | ||
Front Cell Dev Biol MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression.
| ||
J Cell Physiol HIF-1α plays an essential role in BMP9-mediated osteoblast differentiation through the induction of a glycolytic enzyme, PDK1. | ||
Mol Cell Endocrinol miR-122-5p targets GREM2 to protect against glucocorticoid-induced endothelial damage through the BMP signaling pathway. |
