Tested Applications
| Positive WB detected in | A549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse stomach tissue, human placenta tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:175-1:700 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67061-1-Ig targets CNTROB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25447 Product name: Recombinant human CNTROB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC021134 Sequence: MATSADSPSSPLGAEDLLSDSSEPPGLNQVSSEVTSQLYASLRLSRQAEATARAQLYLPSTSPPHEGLDGFAQELSRSLSVGLEKNLKKKDGSKHIFEMESVRGQLQTMLQTSRDTAYRDPLIPGAGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPLNPPDFQGLRDALDSEHTRRKHCERHIQSLQTRVLELQQQLAVAVAADRKKDTMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEEHQAAELTRSKQQETVTRLEQSLSEAMEALNREQESARLQQRERETLEEERQALTLRLEAEQQRCCVLQEERDAARAGQLSEHRELET Predict reactive species |
| Full Name | centrobin, centrosomal BRCA2 interacting protein |
| Calculated Molecular Weight | 903 aa, 101 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC021134 |
| Gene Symbol | CNTROB |
| Gene ID (NCBI) | 116840 |
| RRID | AB_2882371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8N137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| IHC protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| WB protocol for CNTROB antibody 67061-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CNTROB Monoclonal antibody (3D12A4), catalog number 67061-1-Ig, has a total of 2 citations. These citations appear in Glia and Molecular Biology of the Cell. The research fields covered include neuroscience (HDAC inhibition modulating microglial dynamics in ischemic stroke) and cell biology (centriole assembly via CEP152–PLK4 interaction). Both studies used the antibody for immunofluorescence (IF) applications.
| Species | Application | Title |
|---|---|---|
Glia Spatial Transcriptomic Analysis Reveals HDAC Inhibition Modulates Microglial Dynamics to Protect Against Ischemic Stroke in Mice | ||
Mol Biol Cell Binding of CEP152 to PLK4 stimulates kinase activity to promote centriole assembly |
