Tested Applications
| Positive WB detected in | human skeletal muscle tissue, human heart tissue, pig heart tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67082-1-Ig targets Myogenin in WB, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.
| Tested Reactivity | Human, Rat, Pig |
| Cited Reactivity | human, mouse, rabbit, bovine, goat, sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6414 Product name: Recombinant human Myogenin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC053899 Sequence: MELYETSPYFYQEPRFYDGENYLPVHLQGFEPPGYERTELTLSPEAPGPLEDKGLGTPEHCPGQCLPWACKVCKRKSVSVDRRRAATLREKRRLKKVNEAFEALKRSTLLNPNQRLPKVEILRSAIQYIERLQALLSSLNQEERDLRYRGGGGPQPGVPSECSSHSASCSPEWGSALEFSANPGDHLLTADPTDAHNLHSLTSIVDSITVEDVSVAFPDETMPN Predict reactive species |
| Full Name | myogenin (myogenic factor 4) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC053899 |
| Gene Symbol | Myogenin |
| Gene ID (NCBI) | 4656 |
| RRID | AB_2882390 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P15173 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.
Publications
What published studies show
The Myogenin Monoclonal antibody (Cat# 67082-1-Ig) has 20 total citations. These appear in journals including Advanced Science, Journal of Cachexia, Sarcopenia and Muscle, FASEB Journal, Cell Signaling, International Journal of Molecular Sciences, and Frontiers in Molecular Biosciences. The research fields center on skeletal muscle myogenesis and differentiation, muscle regeneration, cancer cachexia-related muscle wasting, circRNA/miRNA regulation of myoblast development, and mitochondrial dynamics in muscle atrophy.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) A Circular RNA Generated from Nebulin (NEB) Gene Splicing Promotes Skeletal Muscle Myogenesis in Cattle as Detected by a Multi-Omics Approach | ||
J Cachexia Sarcopenia Muscle circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop. | ||
J Genet Genomics The ARHGAP10-202aa protein encoded by circARHGAP10 promotes skeletal muscle development and regeneration. | ||
Cell Mol Life Sci Ythdf2 facilitates precursor miR-378/miR-378-5p maturation to support myogenic differentiation | ||
Mol Ther Nucleic Acids Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation. | ||
Life Sci Early-senescent bone marrow mesenchymal stem cells promote C2C12 cell myogenic differentiation by preventing the nuclear translocation of FOXO3. |
