Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67101-1-Ig targets DUSP12 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8241 Product name: Recombinant human DUSP12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-340 aa of BC006286 Sequence: MLEAPGPSDGCELSNPSASRVSCAGQMLEVQPGLYFGGAAAVAEPDHLREAGITAVLTVDSEEPSFKAGPGVEDLWRLFVPALDKPETDLLSHLDRCVAFIGQARAEGRAVLVHCHAGVSRSVAIITAFLMKTDQLPFEKAYEKLQILKPEAKMNEGFEWQLKLYQAMGYEVDTSSAIYKQYRLQKVTEKYPELQNLPQELFAVDPTTVSQGLKDEVLYKCRKCRRSLFRSSSILDHREGSGPIAFAHKRMTPSSMLTTGRQAQCTSYFIEPVQWMESALLGVMDGQLLCPKCSAKLGSFNWYGEQCSCGRWITPAFQIHKNRVDEMKILPVLGSQTGKI Predict reactive species |
| Full Name | dual specificity phosphatase 12 |
| Calculated Molecular Weight | 370 aa, 43 kDa |
| GenBank Accession Number | BC006286 |
| Gene Symbol | DUSP12 |
| Gene ID (NCBI) | 11266 |
| RRID | AB_2882406 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UNI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DUSP12 antibody 67101-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DUSP12 Monoclonal antibody (1G8G10), catalog number 67101-1-Ig, has a total of 2 citations. These appear in Acta Pharm Sin B and Aging (Albany NY). The associated research fields include cardiac regeneration and metabolic reprogramming (via CPT1 inhibition and p38 MAPK signaling), as well as tongue squamous cell carcinoma (TSCC) and anticancer therapy enhancement. Both studies utilized the antibody for Western Blot in mouse samples.
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Reversing metabolic reprogramming by CPT1 inhibition with etomoxir promotes cardiomyocyte proliferation and heart regeneration via DUSP1 ADP-ribosylation-mediated p38 MAPK phosphorylation | ||
Aging (Albany NY) Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin |
