Tested Applications
| Positive WB detected in | pig brain tissue, pig cerebellum tissue, Rabbit brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue, human brain tissue, human colon tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67115-1-Ig targets L1CAM/CD171 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17706 Product name: Recombinant human L1CAM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 794-1143 aa of BC126229 Sequence: QGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFI Predict reactive species |
| Full Name | L1 cell adhesion molecule |
| Calculated Molecular Weight | 1257 aa, 140 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC126229 |
| Gene Symbol | L1CAM |
| Gene ID (NCBI) | 3897 |
| RRID | AB_2882419 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P32004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for L1CAM/CD171 antibody 67115-1-Ig | Download protocol |
| IHC protocol for L1CAM/CD171 antibody 67115-1-Ig | Download protocol |
| WB protocol for L1CAM/CD171 antibody 67115-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The L1CAM/CD171 Monoclonal antibody (3H7B9), catalog number 67115-1-Ig, has 8 total citations. Retrieved citations appear in J Extracell Vesicles, Cell Commun Signal, J Neuroimmune Pharmacol, Brain Behav Immun Health, and Hum Pathol. Research fields include Parkinson's disease biomarkers, spinal cord injury and neural stem cell differentiation, acute ischemic stroke diagnosis, schizophrenia autoantibodies, and FLCN-mutated tumor pathology. Applications cited include WB, IF, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease | ||
Cell Commun Signal Extracellular vesicles from UTX-knockout endothelial cells boost neural stem cell differentiation in spinal cord injury | ||
J Neuroimmune Pharmacol Brain-Derived Exosomal CircRNAs in Plasma Serve as Diagnostic Biomarkers for Acute Ischemic Stroke | ||
Brain Behav Immun Health Identification of autoantibodies against L1CAM in patients with schizophrenia. | ||
Hum Pathol Conventional FLCN-mutated tumor: a retrospective study of 5 cases with emphasis on diagnostic challenges. |
