Tested Applications
| Positive IHC detected in | mouse brain tissue, human hypothalamus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-67115 targets L1CAM/CD171 in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17706 Product name: Recombinant human L1CAM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 794-1143 aa of BC126229 Sequence: QGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFI Predict reactive species |
| Full Name | L1 cell adhesion molecule |
| Calculated Molecular Weight | 1257 aa, 140 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC126229 |
| Gene Symbol | L1CAM |
| Gene ID (NCBI) | 3897 |
| RRID | AB_2918949 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P32004 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Biotin L1CAM/CD171 antibody Biotin-67115 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Biotin-67115 has 2 total citations, published in International Journal of Molecular Sciences (Int J Mol Sci) and Molecular Neurobiology (Mol Neurobiol). Both studies focus on neurodegenerative disease research, specifically using neuronally derived extracellular vesicles to differentiate Parkinson's Disease from Essential Tremor. The antibody was applied in immunoprecipitation (IP) for human samples.
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Neuronally Derived Extracellular Vesicles' Oligomeric and p129-α-Synuclein Levels for Differentiation of Parkinson's Disease from Essential Tremor | ||
Mol Neurobiol Neurofilament Light Chain from Neuronally Derived Extracellular Vesicles in Differentiating Parkinson's Disease from Essential Tremor with Resting Tremor. |
