Biotin-conjugated L1CAM/CD171 Monoclonal antibody

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  IHC staining of mouse brain using Biotin-67115

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hypothalamus using Biotin-67115

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hypothalamus using Biotin-67115

  Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using Biotin-67115 (L1CAM antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

L1CAM/CD171 Monoclonal Antibody for IHC

Cat No. Biotin-67115

Clone No.3H7B9

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IHC

Synonyms

L1CAM, 3H7B9, CAML1, CD171, HSAS

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human hypothalamus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-67115 targets L1CAM/CD171 in IHC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17706

  Product name: Recombinant human L1CAM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 794-1143 aa of BC126229

  Sequence: QGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFI

• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 1257 aa, 140 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC126229
• Gene Symbol: L1CAM
• Gene ID (NCBI): 3897
• RRID: AB_2918949
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P32004
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Int J Mol Sci; Neuronally Derived Extracellular Vesicles' Oligomeric and p129-α-Synuclein Levels for Differentiation of Parkinson's Disease from Essential Tremor; Authors - Costanza Maria Cristiani