Tested Applications
| Positive WB detected in | NCI-H1299 cells, A549 cells, HT-29 cells, THP-1 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67118-1-Ig targets RAB3D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16784 Product name: Recombinant human RAB3D protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-219 aa of BC016471 Sequence: MASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC Predict reactive species |
| Full Name | RAB3D, member RAS oncogene family |
| Calculated Molecular Weight | 219 aa, 24 kDa |
| Observed Molecular Weight | 24-27 kDa |
| GenBank Accession Number | BC016471 |
| Gene Symbol | RAB3D |
| Gene ID (NCBI) | 9545 |
| RRID | AB_2882421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95716 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAB3D antibody 67118-1-Ig | Download protocol |
| IHC protocol for RAB3D antibody 67118-1-Ig | Download protocol |
| WB protocol for RAB3D antibody 67118-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is a 5-star rating for Western Blot. The user tested it on HEK293T cell extracts (10 µg/lane) at a 1:2,500 dilution in 5% BSA blocking buffer, with overnight incubation at 4°C. A goat anti-mouse HRP secondary antibody at 1:10,000 was used for 1 hour at room temperature. The reviewer reported successful detection with clear results, indicating the antibody performs well under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tom (Verified Customer) (11-13-2020) | HEK293T cell extracts (10ug/lane). Primary antibody (1:2500) in block (5% BSA) incubated at 4 degrees overnight. Goat anti-mouse HRP secondary antibody (1:10,000) incubated for 1 hour at RT.
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