Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
67126-1-Ig targets DDR2 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28340 Product name: Recombinant human DDR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 291-398 aa of NM_001014796 Sequence: MFAKGVKIFKEVQCYFRSEASEWEPNAISFPLVLDDVNPSARFVTVPLHHRMASAIKCQYHFADTWMMFSEITFQSDAAMYNNSEALPTSPMAPTTYDPMLKVDDSNT Predict reactive species |
| Full Name | discoidin domain receptor tyrosine kinase 2 |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | NM_001014796 |
| Gene Symbol | DDR2 |
| Gene ID (NCBI) | 4921 |
| RRID | AB_2882426 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16832 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDR2 (Discoidin domain receptor 2) belongs to the receptor tyrosine kinase (RTK) family and is activated by collagen-binding.DDR2 is implicated in several physiological and pathological processes, including wound healing, angiogenesis, ovulation, spermatogenesis, extracellular matrix (ECM) remodeling and fibrosis, and tumor progression (PMID: 25805889). Moreover, DDR2 is prominently present in the fibroblasts, smooth muscle cells, myofibroblasts, and chondrocytes (PMID: 37834343).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDR2 antibody 67126-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis. | ||
Int J Biol Macromol Human type III collagen-derived biomaterial with high antitumor activity inhibits breast cancer cell autophagy, proliferation, and migration through DDR1 |
