Tested Applications
| Positive WB detected in | HepG2 cells, Jurkat cells, K-562 cells, NCI-H1299 cells, PC-3 cells, A549 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67140-1-Ig targets TCF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15734 Product name: Recombinant human TCF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 318-480 aa of BC110580 Sequence: WPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTRCQ Predict reactive species |
| Full Name | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) |
| Calculated Molecular Weight | 654 aa, 68 kDa |
| Observed Molecular Weight | 68-70 kDa |
| GenBank Accession Number | BC110580 |
| Gene Symbol | TCF3 |
| Gene ID (NCBI) | 6929 |
| RRID | AB_2882439 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TCF3 antibody 67140-1-Ig | Download protocol |
| WB protocol for TCF3 antibody 67140-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TCF3 Monoclonal antibody (Catalog No. 67140-1-Ig) has 7 citations. These appear in journals including Journal of Experimental & Clinical Cancer Research, Cell Death Discovery, Cell Proliferation, Molecular Medicine Reports, Cancer Medicine, Reproduction, and Clinical and Translational Oncology. The associated research fields span oncology (hepatoblastoma, colorectal cancer, lung cancer, Wilms' tumor), bone regeneration, and reproductive biology (endometrial stromal cell regulation). Applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res TCF3 activates super-enhancer-driven TRIB2 overexpression to suppress ferroptosis and promote hepatoblastoma proliferation. | ||
Cell Death Discov TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis | ||
Cell Prolif Unexpected Promotion of Bone Regeneration by Inhibition of BMPR1A-Mediated BMP Signalling. | ||
Mol Med Rep Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.
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Cancer Med Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling | ||
Reproduction TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.
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