Tested Applications
| Positive WB detected in | HeLa cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67195-1-Ig targets NUFIP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29040 Product name: Recombinant human NUFIP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 357-695 aa of BC108307 Sequence: SYASKVKENLNKTIQNSSVSPTSSSSSSSSTGETQTQSSSRLSQVPMSALKSVTSANFSNGPVLAGTDGNVYPPGGQPLLTTAANTLTPISSGTDSVLQDMSLTSAAVEQIKTSLFIYPSNMQTMLLSTAQVDLPSQTDQQNLGDIFQNQWGLSFINEPSAGPETVTGKSSEHKVMEVTFQGEYPATLVSQGAEIIPSGTEHPVFPKAYELEKRTSPQVLGSILKSGTTSESGALSLEPSHIGDLQKADTSSQGALVFLSKDYEIESQNPLASPTNTLLGSAKEQRYQRGLERNDSWGSFDLRAAIVYHTKEMESIWNLQKQDPKRIITYNEAMDSPDQ Predict reactive species |
| Full Name | nuclear fragile X mental retardation protein interacting protein 2 |
| Calculated Molecular Weight | 695 aa, 76 kDa |
| Observed Molecular Weight | 76 kDa, 82 kDa |
| GenBank Accession Number | BC108307 |
| Gene Symbol | NUFIP2 |
| Gene ID (NCBI) | 57532 |
| RRID | AB_2882489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q7Z417 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NUFIP2 antibody 67195-1-Ig | Download protocol |
| IHC protocol for NUFIP2 antibody 67195-1-Ig | Download protocol |
| WB protocol for NUFIP2 antibody 67195-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NUFIP2 Monoclonal antibody (2A4E3), catalog number 67195-1-Ig, has 2 total citations. These appear in Nucleic Acids Research (2026) and Neurobiology of Disease (2025). The research fields span neuronal differentiation and DNA methylation regulatory networks, as well as ATXN2L–NUFIP2 protein interactions and polyQ expansion–driven NUFIP2 accumulation in neurodegeneration. Both citations used the antibody for immunofluorescence (IF) in human and mouse samples, respectively.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Molecular interactome of HNRNPU reveals regulatory networks in neuronal differentiation and DNA methylation. | ||
Neurobiol Dis ATXN2L primarily interacts with NUFIP2, the absence of ATXN2L results in NUFIP2 depletion, and the ATXN2-polyQ expansion triggers NUFIP2 accumulation |
Reviews
What customers say
The single review for 67195-1-Ig is highly positive, rated 5 stars. The user tested the antibody for Western Blot on HEK293T cells at a 1:2000 dilution and described it as excellent, praising its strong sensitivity and specificity. Overall, the feedback indicates reliable performance for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sun (Verified Customer) (01-05-2023) | An excellent antibody with great sensitivity and specificity.
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