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WB analysis of pig liver using 67216-1-Ig (same clone as 67216-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)
pig liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis of rat liver using 67216-1-Ig (same clone as 67216-1-PBS)
rat liver tissue were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 67216-1-Ig (same clone as 67216-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 67216-1-Ig (same clone as 67216-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 67216-1-Ig (same clone as 67216-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis of SMMC-7721 using 67216-1-Ig (same clone as 67216-1-PBS)
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
WB analysis using 67216-1-Ig (same clone as 67216-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67216-1-Ig (GCK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67216-1-Ig (GCK antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67216-1-Ig (same clone as 67216-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67216-1-Ig (same clone as 67216-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GCK antibody (67216-1-Ig, Clone: 1C3A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67216-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51666-1
Cytometric bead array sample test of MP51666-1, GCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67216-2-PBS. Detection antibody: 67216-1-PBS.
Cytometric bead array standard curve of MP51666-1
Cytometric bead array standard curve of MP51666-1, GCK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67216-2-PBS. Detection antibody: 67216-1-PBS. Standard:Ag8116. Range: 3.125-400 ng/mL.

GCK Monoclonal antibody, PBS Only (Detector)

Name: GCK Monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 67216-1-PBS

GCK Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Cat No. 67216-1-PBS

Clone No.1C3A3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Synonyms

1C3A3, EC:2.7.1.1, Glucokinase, Hexokinase-4, Hexokinase-D

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67216-1-PBS targets GCK as part of a matched antibody pair:

MP51666-1: 67216-2-PBS capture and 67216-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8116 Product name: Recombinant human GCK protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 157-465 aa of BC001890 Sequence: EDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ Predict reactive species
Full Name	glucokinase (hexokinase 4)
Calculated Molecular Weight	52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC001890
Gene Symbol	GCK
Gene ID (NCBI)	2645
RRID	AB_2882508
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P35557
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.