Tested Applications
| Positive WB detected in | Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IF/ICC detected in | PC-3 cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67276-1-Ig targets NEDD4L in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4565 Product name: Recombinant human NEDD4L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 565-911 aa of BC032597 Sequence: EESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD Predict reactive species |
| Full Name | neural precursor cell expressed, developmentally down-regulated 4-like |
| Calculated Molecular Weight | 854 aa, 96 kDa |
| Observed Molecular Weight | 112 kDa, 125 kDa |
| GenBank Accession Number | BC032597 |
| Gene Symbol | NEDD4L |
| Gene ID (NCBI) | 23327 |
| RRID | AB_2882545 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96PU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NEDD4L antibody 67276-1-Ig | Download protocol |
| WB protocol for NEDD4L antibody 67276-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NEDD4L antibody (Cat# 67276-1-Ig) has 5 total citations across journals including Cell Biology and Toxicology, Translational Oncology, Biochimica et Biophysica Acta Molecular Cell Research, Journal of Virology, and Antioxidants & Redox Signaling. Research fields span vascular endothelial dysfunction and macrophage polarization, ferroptosis in colorectal cancer and diabetic kidney disease, NEDD4L-mediated ubiquitination pathways, and viral immune evasion (HSV-1). Applications include WB and CoIP in human and mouse models.
| Species | Application | Title |
|---|---|---|
Cell Biol Toxicol Exosomal NEDD4L derived from HG+oxLDL-induced vascular endothelial cells accelerates macrophage M1 polarization and oxLDL uptake by ubiquitinating IκBα and PPARγ | ||
Transl Oncol PUM2 inhibits ferroptosis and enhances oxaliplatin resistance in COAD via the NEDD4L/NRF2 axis. | ||
Biochim Biophys Acta Mol Cell Res NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras | ||
J Virol HSV-1 UL56 protein recruits cellular NEDD4-family ubiquitin ligases to suppress CD1d expression and NKT cell function | ||
Antioxid Redox Signal SGK2 Promotes NEDD4L-Mediated ACSL4 Ubiquitination to Inhibit Ferroptosis and Alleviate Diabetic Kidney Disease. |
