Tested Applications
| Positive WB detected in | human skeletal muscle tissue, pig skeletal muscle tissue, mouse skeletal muscle tissue, rabbit skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67299-1-Ig targets MYH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17129 Product name: Recombinant human MYH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC114545 Sequence: MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV Predict reactive species |
| Full Name | myosin, heavy chain 1, skeletal muscle, adult |
| Calculated Molecular Weight | 1939 aa, 223 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC114545 |
| Gene Symbol | MYH1 |
| Gene ID (NCBI) | 4619 |
| RRID | AB_2882563 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12882 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH1 antibody 67299-1-Ig | Download protocol |
| IHC protocol for MYH1 antibody 67299-1-Ig | Download protocol |
| WB protocol for MYH1 antibody 67299-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH1 Monoclonal antibody (1G10H9), catalog number 67299-1-Ig, has 17 citations published across 16 journals including J Cachexia Sarcopenia Muscle, J Biol Chem, Int J Mol Sci, BMC Genomics, Cells, Nutrients, and Aging. The cited research primarily focuses on skeletal muscle biology, muscle fiber typing, muscle atrophy and cachexia, muscle development and regeneration, and myosin heavy chain function, spanning species such as mouse, pig, human, rat, rabbit, and chicken.
| Species | Application | Title |
|---|---|---|
J Cachexia Sarcopenia Muscle Cancer IDO1-Mediated Tryptophan-Kynurenine Metabolic Reprogramming to Drive Skeletal Muscle Atrophy and Cachexia Acceleration. | ||
Int J Biol Macromol LncRNA lncMGR regulates skeletal muscle development and regeneration by recruiting CDK9 and sponging miRNAs | ||
Food Funct Potential nutritional healthy-aging strategy: enhanced protein metabolism by balancing branched-chain amino acids in a finishing pig model. | ||
Cells Ambient Temperature Shapes Skeletal Muscle Growth and Fiber-Type Plasticity in Mice. | ||
Nutrients Sodium Butyrate Alleviates Heat Stress-Induced Oxidative Stress and Skeletal Muscle Homeostasis Disruption by Promoting Autophagy in Mice | ||
Int J Mol Sci Endurance Exercise-Induced Fgf21 Promotes Skeletal Muscle Fiber Conversion through TGF-β1 and p38 MAPK Signaling Pathway |
Reviews
What customers say
The single review for this antibody reports a Western Blot application on renal carcinoma cells, using a 1:100,000 dilution. The reviewer compared normal tissue (N1/N2) against tumor tissue (T1/T2/T3), and rated the product 4 out of 5 stars. Overall, the feedback suggests the antibody performed well for detecting the target in renal carcinoma samples, with clear differentiation between normal and tumor tissues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Moon (Verified Customer) (10-10-2022) | normal tissue-1/2 (N1/N2) tumor tissue-1/2/3 (T1/T2/T3)
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