CoraLite® Plus 488-conjugated MYH1 Monoclonal antibody

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  IF Staining of mouse skeletal muscle using CL488-67299

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite® Plus 488 MYH1 antibody (CL488-67299, Clone: 1G10H9 ) at dilution of 1:200.

MYH1 Monoclonal Antibody for IF-P

Cat No. CL488-67299

Clone No.1G10H9

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, pig

Applications

IF-P

Synonyms

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

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Tested Applications

• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67299 targets MYH1 in IF-P applications and shows reactivity with Human, mouse, pig samples.

• Tested Reactivity: Human, mouse, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17129

  Product name: Recombinant human MYH1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC114545

  Sequence: MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV

• Full Name: myosin, heavy chain 1, skeletal muscle, adult
• Calculated Molecular Weight: 1939 aa, 223 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC114545
• Gene Symbol: MYH1
• Gene ID (NCBI): 4619
• RRID: AB_3084334
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12882
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.