Filter: AllWBIHCIF/ICC

WB analysis using 67313-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis using 67313-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of 4T1 using 67313-1-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 67313-1-Ig
PC-12 cells were subjected to SDS PAGE followed by western blot with 67313-1-Ig (SSRP1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 67313-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67313-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67313-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 67313-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67313-1-Ig (SSRP1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67313-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67313-1-Ig (SSRP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 67313-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SSRP1 antibody (67313-1-Ig, Clone: 3D3H4 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Featured Product
KD/KO Validated

SSRP1 Monoclonal antibody

Name: SSRP1 Monoclonal antibody
Brand: Proteintech
SKU: 67313-1-Ig

SSRP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67313-1-Ig

Clone No.3D3H4

Publications(1)

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3D3H4, Chromatin-specific transcription elongation factor 80 kDa subunit, Facilitates chromatin transcription complex 80 kDa subunit, Facilitates chromatin transcription complex subunit SSRP1, FACT

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®555 CoraLite®594

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, 4T1 cells, NCCIT cells, PC-12 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, NIH/3T3 cells
Positive IHC detected in	human ovary tumor tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IHC	See 1 publications below

Product Information

67313-1-Ig targets SSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8318 Product name: Recombinant human SSRP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 361-709 aa of BC005116 Sequence: YVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE Predict reactive species
Full Name	structure specific recognition protein 1
Calculated Molecular Weight	81 kDa
Observed Molecular Weight	86 kDa
GenBank Accession Number	BC005116
Gene Symbol	SSRP1
Gene ID (NCBI)	6749
RRID	AB_2882573
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q08945
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SSRP1 antibody 67313-1-Ig	Download protocol
IHC protocol for SSRP1 antibody 67313-1-Ig	Download protocol
WB protocol for SSRP1 antibody 67313-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Biomed Pharmacother CBL0137 activates ROS/BAX signaling to promote caspase-3/GSDME-dependent pyroptosis in ovarian cancer cells Authors - Chang Yang KD Validated View Article