Tested Applications
| Positive WB detected in | rat brain tissue, pig brain tissue, mouse brain tissue |
| Positive IHC detected in | rat brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue, mouse kidney tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67339-1-Ig targets SYNPO in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15089 Product name: Recombinant human SYNPO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 336-685 aa of BC146665 Sequence: SPPSYSVLYPSSDPKSSHLKGQAVPASKTGILEESMARRGSRKSMFTFVEKPKVTPNPDLLDLVQTADEKRRQRDQGEVGVEEEPFALGAEASNFQQEPAPRDRASPAAAEEVVPEWASCLKSPRIQAKPKPKPNQNLSEASGKGAELYARRQSRMEKYVIESSSHTPELARCPSPTMSLPSSWKYPTNAPGAFRVASRSPARTPPASLYHGYLPENGVLRPEPTKQPPYQLRPSLFVLSPIKEPAKVSPRAASPAKPSSLDLVPNLPKGALPPSPALPRPSRSSPGLYTSPGQDSLQPTAVSPPYGGDISPVSPSRAWSPRAKQAPRPSFSTRNAGIEAQVWKPSFCFK Predict reactive species |
| Full Name | synaptopodin |
| Calculated Molecular Weight | 929 aa, 99 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC146665 |
| Gene Symbol | SYNPO |
| Gene ID (NCBI) | 11346 |
| RRID | AB_2882597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8N3V7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SYNPO antibody 67339-1-Ig | Download protocol |
| IHC protocol for SYNPO antibody 67339-1-Ig | Download protocol |
| WB protocol for SYNPO antibody 67339-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SYNPO Monoclonal antibody (Cat# 67339-1-Ig) has 9 citations spanning journals including Advanced Science, Cell Death & Disease, Molecular Medicine, Cell & Molecular Life Sciences, International Immunopharmacology, Inflammation, Frontiers in Cell and Developmental Biology, FASEB Journal, and Inflammation Research. Research fields center on diabetic kidney disease, podocyte injury, renal fibrosis, autophagy, and preeclampsia, with applications in WB, IF, and IHC across mouse and human samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) ANGPTL4 Exacerbates Renal Injury in Diabetic Kidney Disease by Impairing Podocyte Lipophagy via Compromised Lysosomal Degradative Function. | ||
Cell Death Dis Sestrin2 remedies podocyte injury via orchestrating TSP-1/TGF-β1/Smad3 axis in diabetic kidney disease. | ||
Mol Med REDD1 deficiency alleviates podocyte PANoptosis and restores autophagy in diabetic kidney disease. | ||
Cell Mol Life Sci Extracellular vesicle-derived TMEM106A participated in podocyte injury via EGR1 in preeclampsia. | ||
Int Immunopharmacol Long noncoding RNA ENST00000458139 promotes mitochondrial dysfunction and apoptosis of podocytes in diabetic kidney disease through binding with SNRPA1. | ||
Inflammation Canagliflozin Attenuates Podocyte Inflammatory Injury through Suppressing the TXNIP/NLRP3 Signaling Pathway in Diabetic Kidney Disease Mice |
