SYNPO Monoclonal antibody, PBS Only

• 

  WB analysis of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67339-1-Ig (same clone as 67339-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67339-1-Ig (same clone as 67339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 67339-1-Ig (same clone as 67339-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67339-1-Ig (same clone as 67339-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

SYNPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 67339-1-PBS

Clone No.1E1B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Synaptopodin, 1E1B1, KIAA1029, snaptopodin

---

Product Information

67339-1-PBS targets SYNPO in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15089

  Product name: Recombinant human SYNPO protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 336-685 aa of BC146665

  Sequence: SPPSYSVLYPSSDPKSSHLKGQAVPASKTGILEESMARRGSRKSMFTFVEKPKVTPNPDLLDLVQTADEKRRQRDQGEVGVEEEPFALGAEASNFQQEPAPRDRASPAAAEEVVPEWASCLKSPRIQAKPKPKPNQNLSEASGKGAELYARRQSRMEKYVIESSSHTPELARCPSPTMSLPSSWKYPTNAPGAFRVASRSPARTPPASLYHGYLPENGVLRPEPTKQPPYQLRPSLFVLSPIKEPAKVSPRAASPAKPSSLDLVPNLPKGALPPSPALPRPSRSSPGLYTSPGQDSLQPTAVSPPYGGDISPVSPSRAWSPRAKQAPRPSFSTRNAGIEAQVWKPSFCFK

• Full Name: synaptopodin
• Calculated Molecular Weight: 929 aa, 99 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC146665
• Gene Symbol: SYNPO
• Gene ID (NCBI): 11346
• RRID: AB_2882597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8N3V7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)