Tested Applications
| Positive WB detected in | SGC-7901 cells, A549 cells, HeLa cells, HepG2 cells, HL-60 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67398-1-Ig targets Caspase 4 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29488 Product name: Recombinant human Caspase 4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-377 aa of BC017839 Sequence: MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN Predict reactive species |
| Full Name | caspase 4, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 377 aa, 43 kDa |
| Observed Molecular Weight | 43-48 kDa |
| GenBank Accession Number | BC017839 |
| Gene Symbol | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_2882641 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49662 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
| IHC protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
| WB protocol for Caspase 4 antibody 67398-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Caspase 4 Monoclonal antibody (1D1E12), catalog number 67398-1-Ig, has 7 citations spanning journals including Adv Sci, EMBO J, ACS Chem Neurosci, Mol Carcinog, Biochem Biophys Rep, Life Sci Alliance, and Hum Cell. Research fields covered include anti-tumor immunity, inflammasome and pyroptosis, neuroinflammation, lung squamous cell carcinoma, allergic rhinitis with autophagy, caspase biology, and glioma prognosis. Applications cited are primarily WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Irradiated Tumor Cell-Derived Microparticles Activate Systemic Anti-Tumor Immunity via the STING/NLRP3/GSDMD Axis in Neutrophils. | ||
EMBO J Cardiolipin inhibits the non-canonical inflammasome by preventing LPS binding to caspase-4/11. | ||
ACS Chem Neurosci Hypoxanthine Produces Rapid Antidepressant Effects by Suppressing Inflammation in Serum and Hippocampus | ||
Mol Carcinog Pyroptosis-related gene GSDMC indicates poor prognosis and promotes tumor progression by activating the AKT/mTOR pathway in lung squamous cell carcinoma | ||
Biochem Biophys Rep HMGB1 impairs nasal mucosa epithelial barrier function in allergic rhinitis by promoting BECN1-mediating autophagy. | ||
Life Sci Alliance Caspase-4 dimerisation and D289 auto-processing elicit an interleukin-1β-converting enzyme |
Reviews
What customers say
Both reviews (5/5 stars) are for Western Blot applications. One reviewer used a 1:2000 dilution on human epithelial and myeloid cells, praising the antibody's efficiency as a primary antibody and its ability to detect cleaved Caspase-4, which they noted is challenging with other antibodies. They cautioned that performance may decrease on membranes previously treated with peroxide. The second reviewer used a 1:1000 dilution on THP-1 cells and gave a full rating without additional comments. Overall, users report strong performance and reliable detection in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dave (Verified Customer) (06-13-2022) | This is a great antibody. Work very efficiently if you use it as the first antibody (worse less well if your membrane has already been deactivated with Peroxide). This product also detect efficiently cleaved Caspase-4 (which can be very challenging with other antibodies)
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Aldona (Verified Customer) (10-29-2021) | I have no comments
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