Filter: AllWBIHCIF/ICCCytometric bead array sample testCytometric bead array

WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.
WB analysis using 67470-1-Ig (same clone as 67470-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67470-1-Ig (PGAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67470-1-Ig (same clone as 67470-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67470-1-Ig (PGAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 67470-1-Ig (same clone as 67470-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGAM1 antibody (67470-1-Ig, Clone: 2H2A9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67470-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51572-1
Cytometric bead array sample test of MP51572-1, PGAM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67470-1-PBS. Detection antibody: 67470-2-PBS.
Cytometric bead array standard curve of MP51572-1
Cytometric bead array standard curve of MP51572-1, PGAM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67470-1-PBS. Detection antibody: 67470-2-PBS. Standard:Ag9250. Range: 0.078-10 ng/mL.

Featured Product
KD/KO Validated

PGAM1 Monoclonal antibody, PBS Only (Capture)

Name: PGAM1 Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 67470-1-PBS

PGAM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67470-1-PBS

Clone No.2H2A9

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BPG dependent PGAM 1, PGAM B, PGAM1, PGAMA, Phosphoglycerate mutase 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

67470-1-PBS targets PGAM1 as part of a matched antibody pair:

MP51572-1: 67470-1-PBS capture and 67470-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag9250 Product name: Recombinant human PGAM1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-254 aa of BC011678 Sequence: MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK Predict reactive species
Full Name	phosphoglycerate mutase 1 (brain)
Calculated Molecular Weight	254 aa, 29 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC011678
Gene Symbol	PGAM1
Gene ID (NCBI)	5223
RRID	AB_2882699
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P18669
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PGAM1(phosphoglycerate mutase 1) is also named as PGAMA,PGAM-B and belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The homodimer PGAM1 is expressed mainly in liver, kidney, brain and overexpressed in a variety of human cancers, including breast carcinoma, colorectal cancer, lung cancer, prostate cancer, oral squamous cell carcinoma, esophageal squamous cell carcinomas and also associated with certain virus infection. PGAM1 could be developed as a useful diagnostic biomarker, as well as a potential therapeutic target for hepatocellular carcinoma (PMID:20403181). This antibody may also recognize PGAM2 and PGAM4 due to the high homology.