Tested Applications
| Positive WB detected in | LNCaP cells, HAP1 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, HepG2 cells |
| Positive IP detected in | HAP1 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HAP1 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67499-1-Ig targets PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4727 Product name: Recombinant human PRDX6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC035857 Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP Predict reactive species |
| Full Name | peroxiredoxin 6 |
| Calculated Molecular Weight | 224 aa, 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC035857 |
| Gene Symbol | PRDX6 |
| Gene ID (NCBI) | 9588 |
| RRID | AB_2882723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30041 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRDX6 antibody 67499-1-Ig | Download protocol |
| IHC protocol for PRDX6 antibody 67499-1-Ig | Download protocol |
| WB protocol for PRDX6 antibody 67499-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PRDX6 antibody (Cat# 67499-1-Ig) has 3 total citations published in Redox Reports, Reproductive Biology and Endocrinology, and iScience. Associated research fields include ferroptosis and sepsis-associated acute kidney injury, oxidative stress in asthenozoospermic sperm, and microprotein identification in cancer cell fitness. Applications cited include Western blot and immunofluorescence, with reactivity in mouse, human, and unspecified species.
| Species | Application | Title |
|---|---|---|
Redox Rep Sirtuin 5-mediated desuccinylation of PRDX6 inhibits ferroptosis and alleviates sepsis-associated acute kidney injury. | ||
Reprod Biol Endocrinol Semenogelin I-derived peptide SgI-52 binds membrane Peroxiredoxin 6 and induces oxidative stress in asthenozoospermic sperm. | ||
iScience A large-scale sORF screen identifies putative microproteins involved in cancer cell fitness |
