Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67500-1-Ig targets RAN in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30034 Product name: Recombinant human RAN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-216 aa of BC004272 Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL Predict reactive species |
| Full Name | RAN, member RAS oncogene family |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC004272 |
| Gene Symbol | RAN |
| Gene ID (NCBI) | 5901 |
| RRID | AB_2882724 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62826 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAN antibody 67500-1-Ig | Download protocol |
| IHC protocol for RAN antibody 67500-1-Ig | Download protocol |
| WB protocol for RAN antibody 67500-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAN Monoclonal antibody (1B11E1), catalog number 67500-1-Ig, has a total of 3 citations. These appear in Journal of Advanced Research, Advanced Science, and Lipids in Health and Disease. The associated research fields include virology and interferon signaling, epigenetic regulation of hepatocellular carcinoma, and lipid metabolism-related immunosuppression in diffuse large B-cell lymphoma.
| Species | Application | Title |
|---|---|---|
J Adv Res Voxtalisib inhibits enterovirus 71 replication by downregulating host RAN and restoring IFN-STAT signaling | ||
Adv Sci (Weinh) H3K36me3-Guided m6A Modification of Oncogenic L1CAM-AS1 Drives Macrophage Polarization and Immunotherapy Resistance in Hepatocellular Carcinoma | ||
Lipids Health Dis A novel lipid metabolism-based risk model associated with immunosuppressive mechanisms in diffuse large B-cell lymphoma |
