RAN Monoclonal antibody, PBS Only

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  WB analysis using 67500-1-Ig (same clone as 67500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67500-1-Ig (RAN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67500-1-Ig (same clone as 67500-1-PBS)

  WB result of RAN antibody (67500-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAN transfected HEK-293 cells. This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67500-1-Ig (RAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67500-1-Ig (same clone as 67500-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using RAN antibody (67500-1-Ig, Clone: 1B11E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67500-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RAN Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67500-1-PBS

Clone No.1B11E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

ARA24, 1B11E1, Androgen receptor-associated protein 24, EC:3.6.5.-, GTP binding nuclear protein Ran

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Product Information

67500-1-PBS targets RAN in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30034

  Product name: Recombinant human RAN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-216 aa of BC004272

  Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL

• Full Name: RAN, member RAS oncogene family
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC004272
• Gene Symbol: RAN
• Gene ID (NCBI): 5901
• RRID: AB_2882724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62826
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RAN is also named as ARA24, Ras-like protein TC4 and GTPase Ran, and belongs to the small GTPase superfamily family and the Ran family. GTPase Ran switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PMID:7819259, PMID:32528950). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PMID:10408446, PMID:29040603, PMID:32528950).