Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, U2OS cells, LNCaP cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67525-1-Ig targets IPO7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27257 Product name: Recombinant human IPO7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 879-1016 aa of NM_006391.2 Sequence: MHAEHENDSDDDDEAEDDDETEELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQTIQNRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAHESKMIEKHGG Predict reactive species |
| Full Name | importin 7 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | NM_006391.2 |
| Gene Symbol | IPO7 |
| Gene ID (NCBI) | 10527 |
| RRID | AB_2882745 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95373 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IPO7 antibody 67525-1-Ig | Download protocol |
| WB protocol for IPO7 antibody 67525-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IPO7 Monoclonal antibody (1E7C12), catalog number 67525-1-Ig, has 1 total citation. It appears in Experimental Neurology (IF 4.8, 2025). The cited study investigated how SDF2L1 downregulation mediates high glucose–induced Schwann cell dysfunction through inhibition of TFEB and CREB nuclear import via KPNA3, placing it in the fields of neurology, diabetic neuropathy, and cellular signaling. The antibody was used for Western blot in rat samples.
| Species | Application | Title |
|---|---|---|
Exp Neurol SDF2L1 downregulation mediates high glucose-caused Schwann cell dysfunction by inhibiting nuclear import of TFEB and CREB via KPNA3 |
