Tested Applications
| Positive WB detected in | MCF-7 cells, COLO 320 cells, PC-3 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67527-1-Ig targets GSDMB in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28332 Product name: Recombinant human GSDMB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-385 aa of BC025682 Sequence: MFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLMDILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIKISENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFWSQISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMSAGLDIHFRGKTKSFPEGKSLGSEDSRNMKEKLEDMESVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSEVLISRELHMEDSDKPLLSSLFNAAGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGTLPLLKDQVKSVMEQNWDELASSPPDMDYDPE Predict reactive species |
| Full Name | gasdermin B |
| Calculated Molecular Weight | 411 aa, 47 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC025682 |
| Gene Symbol | GSDMB |
| Gene ID (NCBI) | 55876 |
| RRID | AB_2882746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8TAX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GSDMB antibody 67527-1-Ig | Download protocol |
| WB protocol for GSDMB antibody 67527-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GSDMB Monoclonal antibody (2F12C4), catalog number 67527-1-Ig, has 2 total citations. These appear in Biochemical Pharmacology (2026) and Archives of Biochemistry and Biophysics (2024). The associated research fields include cervical cancer biology—specifically mitochondrial calcium overload, apoptosis, and GSDME-mediated pyroptosis—and abdominal aortic aneurysm pathogenesis involving macrophage non-canonical pyroptosis. Both studies utilized WB and IF applications in human samples.
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Overexpression of GJB1-13k induces mitochondrial calcium overload and triggers apoptosis and GSDME-mediated pyroptosis in cervical cancer. | ||
Arch Biochem Biophys GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis |
