CoraLite® Plus 488-conjugated GSDMB Monoclonal antibody

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  IF Staining of HepG2 using CL488-67527

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GSDMB antibody (CL488-67527, Clone: 2F12C4 ) at dilution of 1:200.

GSDMB Monoclonal Antibody for IF/ICC

Cat No. CL488-67527

Clone No.2F12C4

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

2F12C4, gasdermin B, Gasdermin like protein, Gasdermin-B, Gasdermin-B, C-terminal

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67527 targets GSDMB in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28332

  Product name: Recombinant human GSDMB protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-385 aa of BC025682

  Sequence: MFSVFEEITRIVVKEMDAGGDMIAVRSLVDADRFRCFHLVGEKRTFFGCRHYTTGLTLMDILDTDGDKWLDELDSGLQGQKAEFQILDNVDSTGELIVRLPKEITISGSFQGFHHQKIKISENRISQQYLATLENRKLKRELPFSFRSINTRENLYLVTETLETVKEETLKSDRQYKFWSQISQGHLSYKHKGQREVTIPPNRVLSYRVKQLVFPNKETMSAGLDIHFRGKTKSFPEGKSLGSEDSRNMKEKLEDMESVLKDLTEEKRKDVLNSLAKCLGKEDIRQDLEQRVSEVLISRELHMEDSDKPLLSSLFNAAGVLVEARAKAILDFLDALLELSEEQQFVAEALEKGTLPLLKDQVKSVMEQNWDELASSPPDMDYDPE

• Full Name: gasdermin B
• Calculated Molecular Weight: 411 aa, 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC025682
• Gene Symbol: GSDMB
• Gene ID (NCBI): 55876
• RRID: AB_2934547
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8TAX9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Taylor (Verified Customer) (12-02-2025)

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• Applications: Western Blot
• Cell Tissue Type: Human Monocytes