Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, A549 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, rat stomach tissue, rat testis tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67539-1-Ig targets CCT8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30082 Product name: Recombinant human CCT8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 146-497 aa of BC012584 Sequence: FNVDNIRVCKILGSGISSSSVLHGMVFKKETEGDVTSVKDAKIAVYSCPFDGMITETKGTVLIKTAEELMNFSKGEENLMDAQVKAIADTGANVVVTGGKVADMALHYANKYNIMLVRLNSKWDLRRLCKTVGATALPRLTPPVLEEMGHCDSVYLSEVGDTQVVVFKHEKEDGAISTIVLRGSTDNLMDDIERAVDDGVNTFKVLTRDKRLVPGGGATEIELAKQITSYGETCPGLEQYAIKKFAEAFEAIPRALAENSGVKANEVISKLYAVHQEGNKNVGLDIEAEVPAVKDMLEAGILDTYLGKYWAIKLATNAAVTVLRVDQIIMAKPAGGPKPPSGKKDWDDDQND Predict reactive species |
| Full Name | chaperonin containing TCP1, subunit 8 (theta) |
| Calculated Molecular Weight | 548 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC012584 |
| Gene Symbol | CCT8 |
| Gene ID (NCBI) | 10694 |
| RRID | AB_2882758 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Dysregulation of CCT8 has been linked to proliferation and invasion of tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCT8 antibody 67539-1-Ig | Download protocol |
| IHC protocol for CCT8 antibody 67539-1-Ig | Download protocol |
| WB protocol for CCT8 antibody 67539-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCT8 Monoclonal antibody (Cat# 67539-1-Ig) has 5 citations published in Nature, Cell (×2), J Cancer, and Medicine (Baltimore). Research fields span deep spatial proteomics via tissue expansion, aggrephagy-mediated protein aggregate clearance, chaperonin assembly quality control, CCT8-driven cell migration and tumor metastasis in lung adenocarcinoma, and chaperonin subunit genes as prognostic biomarkers correlated with Th2 cell infiltration in lung adenocarcinoma. Applications include IF, WB, IP, and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Medicine (Baltimore) Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study |
