Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67541-1-Ig targets RBM8A,Y14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6800 Product name: Recombinant human RBM8A,Y14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-174 aa of BC017088 Sequence: MADVLDLHEAGGEDFAMDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQDGDEPGPQRSVEGWILFVTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNGQDLMGQPISVDWCFVRGPPKGKRRGGRRRSRSPDRRRR Predict reactive species |
| Full Name | RNA binding motif protein 8A |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC017088 |
| Gene Symbol | RBM8A |
| Gene ID (NCBI) | 9939 |
| RRID | AB_2882760 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y5S9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM8A,Y14 antibody 67541-1-Ig | Download protocol |
| IHC protocol for RBM8A,Y14 antibody 67541-1-Ig | Download protocol |
| WB protocol for RBM8A,Y14 antibody 67541-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RBM8A, Y14 Monoclonal antibody (1F5G1), catalog number 67541-1-Ig, has 1 total citation. It appears in Nucleic Acids Research (2022), in a study using an RNAi screen of RNA helicases to identify eIF4A3 as a regulator of embryonic stem cell identity. The research field encompasses RNA biology and stem cell gene regulation. The antibody was applied in Western blot (WB) with mouse species in this publication.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity |
