PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67562-1-Ig

Clone No.3G8C1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3G8C1, EC:2.7.11.1, p21 activated kinase 6, p21-activated kinase 6, PAK 5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBIHCIF/ICC

WB analysis of K-562 using 67562-1-Ig
K-562 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 67562-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 67562-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 67562-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 67562-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 67562-1-Ig
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 67562-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PAK6 antibody (67562-1-Ig, Clone: 3G8C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells
Positive IHC detected in	human pancreas cancer tissue, mouse kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67562-1-Ig targets PAK6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag27692 Product name: Recombinant human PAK6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 97-262 aa of BC035596 Sequence: SNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFR Predict reactive species
Full Name	p21 protein (Cdc42/Rac)-activated kinase 6
Calculated Molecular Weight	681 aa, 75 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC035596
Gene Symbol	PAK6
Gene ID (NCBI)	56924
RRID	AB_2882776
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9NQU5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).

Protocols

Product Specific Protocols
IF protocol for PAK6 antibody 67562-1-Ig	Download protocol
IHC protocol for PAK6 antibody 67562-1-Ig	Download protocol
WB protocol for PAK6 antibody 67562-1-Ig	Download protocol