PAK6 Monoclonal antibody, PBS Only

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  WB analysis of K-562 using 67562-1-Ig (same clone as 67562-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67562-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67562-1-Ig (same clone as 67562-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67562-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 67562-1-Ig (same clone as 67562-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PAK6 antibody (67562-1-Ig, Clone: 3G8C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67562-1-PBS in a different storage buffer formulation.

PAK6 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67562-1-PBS

Clone No.3G8C1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

3G8C1, EC:2.7.11.1, p21 activated kinase 6, p21-activated kinase 6, PAK 5

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Product Information

67562-1-PBS targets PAK6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27692

  Product name: Recombinant human PAK6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 97-262 aa of BC035596

  Sequence: SNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFR

• Full Name: p21 protein (Cdc42/Rac)-activated kinase 6
• Calculated Molecular Weight: 681 aa, 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC035596
• Gene Symbol: PAK6
• Gene ID (NCBI): 56924
• RRID: AB_2882776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NQU5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).