Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67680-1-Ig targets NXT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30039 Product name: Recombinant human NXT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-140 aa of BC000759 Sequence: MASVDFKTYVDQACRAAEEFVNVYYTTMDKRRRLLSRLYMGTATLVWNGNAVSGQESLSEFFEMLPSSEFQISVVDCQPVHDEATPSQTTVLVVICGSVKFEGNKQRDFNQNFILTAQASPSNTVWKIASDCFRFQDWAS Predict reactive species |
| Full Name | NTF2-like export factor 1 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC000759 |
| Gene Symbol | NXT1 |
| Gene ID (NCBI) | 29107 |
| RRID | AB_2882873 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NXT1 is located in the nuclear envelope and is homologous to nuclear transport factor 2. NXT1 functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. NXT1 also heterodimerizes with Tap protein and regulates the ability of Tap protein to mediate nuclear mRNA export.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NXT1 antibody 67680-1-Ig | Download protocol |
| WB protocol for NXT1 antibody 67680-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NXT1 Monoclonal antibody (2C10A2), catalog number 67680-1-Ig, has 3 total citations. These appear in iScience, Turkish Journal of Gastroenterology, and NAR Molecular Medicine. The associated research fields include human pluripotent stem cell biology, immune escape in hepatocellular carcinoma, and viral pathogenesis involving Zika and dengue viruses. All citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. | ||
Turk J Gastroenterol Immune Escape-Related Gene NXT1 as a Potential Prognostic and Therapeutic Target in Hepatocellular Carcinoma.
| ||
NAR Mol Med Zika and dengue viruses differentially modulate host mRNA processing factors defining its virulence |
