Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, Jurkat cells, HEK-293 cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67690-1-Ig targets NDUFB8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6569 Product name: Recombinant human NDUFB8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-186 aa of BC000466 Sequence: MAVARAGVLGVQWLQRASRNVMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPDRSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTSPTPVSWHVMCMQLFGFLAFMIFMCWVGDVYPVYQPVGPKQYPYNNLYLERGGDPSKEPERVVHYEI Predict reactive species |
| Full Name | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 18-22 kDa |
| GenBank Accession Number | BC000466 |
| Gene Symbol | NDUFB8 |
| Gene ID (NCBI) | 4714 |
| RRID | AB_2882883 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O95169 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFB8 antibody 67690-1-Ig | Download protocol |
| WB protocol for NDUFB8 antibody 67690-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDUFB8 Monoclonal antibody (3B12C6), catalog number 67690-1-Ig, has 14 citations published across journals including J Immunother Cancer, Phytomedicine, Sci Total Environ, J Transl Med, Biochem Pharmacol, Eur J Pharmacol, Front Endocrinol, Front Aging Neurosci, Microbiol Spectr, and Mol Biol Rep. Research fields span oncology (renal cell carcinoma, glioma), mitochondrial biology, metabolic diseases (diabetes, NAFLD), neurology (neurodevelopment, cognitive decline), nephrology (fibrosis, ischemia-reperfusion), reproductive endocrinology (PCOS), and muscle development.
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Targeting oxidative phosphorylation to increase the efficacy of immune-combination therapy in renal cell carcinoma | ||
Phytomedicine Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics | ||
J Orthop Translat Methylene blue restores NAD(+)/NADH homeostasis to attenuate Achilles tendinopathy via activating AIF/Mitochondrial respiratory chain complex I. | ||
Sci Total Environ Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects | ||
J Transl Med DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway | ||
Phytother Res Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels. |
Reviews
What customers say
The single review for 67690-1-Ig is from a researcher who used the antibody for Western Blot at a 1:8000 dilution on HepG2 cells, mouse liver, and mouse brain tissues. They rated it 4 out of 5 stars, describing it as a "good and strong antibody" while noting the presence of some non-specific bands. Overall, the feedback is positive regarding signal strength, with a minor caveat about background specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (06-17-2021) | Good and strong antibody, but with some non-specific bands
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