RUVBL1 Monoclonal antibody

RUVBL1 Monoclonal Antibody for WB, ELISA

Cat No. 67750-1-Ig

Clone No. 1F2D9

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Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, ELISA
For IF or FC, select conjugated version.

Synonyms

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP interacting protein, 49 kDa TBP-interacting protein, EC:3.6.4.12, ECP 54

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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WB analysis using 67750-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 67750-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67750-1-Ig (RUVBL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

U2OS cells, HeLa cells, HT-1080 cells, HCT 116 cells, K-562 cells, A549 cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67750-1-Ig targets RUVBL1 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag16688 Product name: Recombinant human RUVBL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-214 aa of BC002993 Sequence: MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFD Predict reactive species
Full Name	RuvB-like 1 (E. coli)
Calculated Molecular Weight	456 aa, 50 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC002993
Gene Symbol	RUVBL1
Gene ID (NCBI)	8607
RRID	AB_2918519
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9Y265
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198].