CoraLite® Plus 488-conjugated RUVBL1 Monoclonal antibody

RUVBL1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1


Human, rat


IF, FC (Intra)


CoraLite® Plus 488 Fluorescent Dye



Cat no : CL488-67750


49 kDa TBP interacting protein, ECP 54, ECP54, INO80 complex subunit H, INO80H, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, RuvB like 1, RuvB like 1 (E. coli), RUVBL1, RVB1, TAP54 alpha, TIH1, TIP49, TIP49A

Tested Applications

Positive IF detected inLNCaP cells
Positive FC detected inHEK-293 cells

Recommended dilution

Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67750 targets RUVBL1 in IF, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RUVBL1 fusion protein Ag16688
Full Name RuvB-like 1 (E. coli)
Calculated Molecular Weight 456 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC002993
Gene Symbol RUVBL1
Gene ID (NCBI) 8607
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RUVBL1 is a member of the AAA (ATPase associated with diverse cellular activities) family of proteins that is associated with several chromatin-remodelling complexes and has important roles in transcriptional regulation, the DNA damage response, telomerase activity, snoRNP assembly, cellular transformation and cancer metastasis[PMID: 21617703]. It consists of 456 amino acids (50 kDa) and interacts with TATA-binding protein (TBP), which is a central component for transcriptional regulation by forming complexes with various transcription regulators. RUVBL1 is a component of a large nuclear protein complex, possibly the RNA polymerase II holoenzyme. RUVBL1 is also highly homologous to RuvB proteins that function as a DNA helicase promote branch migration of the Holliday junction[PMID: 9588198].


Product Specific Protocols
IF protocol for CL Plus 488 RUVBL1 antibody CL488-67750Download protocol
FC protocol for CL Plus 488 RUVBL1 antibody CL488-67750Download protocol
Standard Protocols
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