Filter: AllWBIPIHCIF-PCytometric bead array sample testCytometric bead array

WB analysis using 67792-1-Ig (same clone as 67792-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
WB analysis of MDCK using 67792-1-Ig (same clone as 67792-1-PBS)
MDCK cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 67792-1-Ig (same clone as 67792-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67792-1-Ig (SERBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)
IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 67792-1-Ig (same clone as 67792-1-PBS)
IP result of anti-SERBP1 (IP:67792-1-Ig, 5ug; Detection:67792-1-Ig 1:2000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67792-1-Ig (SERBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67792-1-Ig (same clone as 67792-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SERBP1 antibody (67792-1-Ig, Clone: 1D4H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67792-1-PBS in a different storage buffer formulation.
Cytometric bead array sample test of MP51733-1
Cytometric bead array sample test of MP51733-1, SERBP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67792-1-PBS. Detection antibody: 67792-2-PBS.
Cytometric bead array standard curve of MP51733-1
Cytometric bead array standard curve of MP51733-1, SERBP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67792-1-PBS. Detection antibody: 67792-2-PBS. Standard:Ag30202. Range: 3.125-400 ng/mL.

SERBP1 Monoclonal antibody, PBS Only (Capture)

Name: SERBP1 Monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 67792-1-PBS

SERBP1 Monoclonal Antibody for WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

Cat No. 67792-1-PBS

Clone No.1D4H1

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

1D4H1, CGI-55, CHD3IP, HABP4L, PAI RBP1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67792-1-PBS targets SERBP1 as part of a matched antibody pair:

MP51733-1: 67792-1-PBS capture and 67792-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat, pig, canine
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag30202 Product name: Recombinant human SERBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 108-408 aa of BC008045 Sequence: RVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA Predict reactive species
Full Name	SERPINE1 mRNA binding protein 1
Calculated Molecular Weight	45 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC008045
Gene Symbol	SERBP1
Gene ID (NCBI)	26135
RRID	AB_2918556
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q8NC51
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).