SERBP1 Monoclonal antibody

SERBP1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, canidae, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D4H1

Cat no : 67792-1-Ig

Synonyms

CGI 55, CHD3IP, DKFZp564M2423, FLJ90489, HABP4L, PAI RBP1, PAI1 RNA binding protein 1, PAIRBP1, SERBP1



Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, MDCK cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67792-1-Ig targets SERBP1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat, canidae, pig samples.

Tested Reactivity Human, Mouse, Rat, canidae, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SERBP1 fusion protein Ag30202
Full Name SERPINE1 mRNA binding protein 1
Calculated molecular weight 45 kDa
Observed molecular weight50 kDa
GenBank accession numberBC008045
Gene symbol SERBP1
Gene ID (NCBI) 26135
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs).

Protocols

Product Specific Protocols
WB protocol for SERBP1 antibody 67792-1-IgDownload protocol
IHC protocol for SERBP1 antibody 67792-1-IgDownload protocol
IF protocol for SERBP1 antibody 67792-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols