Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, NCI-H1299 cells, HeLa cells, THP-1 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67861-1-Ig targets CBS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7008 Product name: Recombinant human CBS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 206-551 aa of BC000440 Sequence: VAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLVASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYEVEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGSTVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFLKEEDLTEKKPWWWHLRVQELGLSAPLTVLPTITCGHTIEILREKGFDQAPVVDEAGVILGMVTLGNMLSSLLAGKVQPSDQVGKVIYKQFKQIRLTDTLGRLSHILEMDHFALVVHEQIQYHSTGKSSQRQMVFGVVTAIDLLNFVAAQERDQK Predict reactive species |
| Full Name | cystathionine-beta-synthase |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 61-63 kDa |
| GenBank Accession Number | BC000440 |
| Gene Symbol | CBS |
| Gene ID (NCBI) | 875 |
| RRID | AB_2918619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35520 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CBS antibody 67861-1-Ig | Download protocol |
| IHC protocol for CBS antibody 67861-1-Ig | Download protocol |
| WB protocol for CBS antibody 67861-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CBS Monoclonal antibody (1E4F10), catalog number 67861-1-Ig, has 9 total citations. These appear in journals including Nature Communications, Journal of Nanobiotechnology, International Journal of Biological Macromolecules, Biochemical Pharmacology, Biomedicine & Pharmacotherapy, Biomedicines, FEBS Journal, Open Life Sciences, and Free Radical Biology and Medicine. The research fields span colorectal cancer nanotherapy, rheumatoid arthritis, homocystinuria, preeclampsia, pulmonary arterial hypertension, schizophrenia, neurovascular biology, and mitochondrial bioenergetics.
| Species | Application | Title |
|---|---|---|
Nat Commun In situ valence-transited arsenic nanosheets for multi-modal therapy of colorectal cancer | ||
J Nanobiotechnology NaHS@Cy5@MS@SP nanoparticles improve rheumatoid arthritis by inactivating the Hedgehog signaling pathway through sustained and targeted release of H2S into the synovium | ||
Int J Biol Macromol Structure-activity relationship of S-adenosylmethionine analogs as pharmacological chaperones for cystathionine beta-synthase-deficient homocystinuria.
| ||
Biochem Pharmacol Givinostat rescues folding of cystathionine beta-synthase and ameliorates murine homocystinuria | ||
Biomed Pharmacother Hydrogen sulfide ameliorated preeclampsia via suppression of toll-like receptor 4-activated inflammation in the rostral ventrolateral medulla of rats. | ||
Biomedicines H(2)S Donor Therapy Reverses Established Pulmonary Arterial Hypertension and Pulmonary Vascular Structural Remodeling in Rats. |
