Tested Applications
| Positive WB detected in | K-562 cells, HEK-293T cells, HeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells, HEK-293 cells, HSC-T6 cells, 4T1 cells |
| Positive IHC detected in | human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67863-1-Ig targets HSP47/SERPINH1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30999 Product name: Recombinant human SERPINH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-418 aa of BC014623 Sequence: MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC014623 |
| Gene Symbol | HSP47/SERPINH1 |
| Gene ID (NCBI) | 871 |
| RRID | AB_2918621 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P50454 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HSP47/SERPINH1 antibody 67863-1-Ig | Download protocol |
| IF protocol for HSP47/SERPINH1 antibody 67863-1-Ig | Download protocol |
| IHC protocol for HSP47/SERPINH1 antibody 67863-1-Ig | Download protocol |
| WB protocol for HSP47/SERPINH1 antibody 67863-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Hsp47 (SERPINH1) antibody, catalog number 67863-1-Ig, has 4 total citations. These appear in journals including ACS Applied Materials & Interfaces, iScience, Journal of Proteome Research, and eLife. The associated research fields span pyroptosis in amyotrophic lateral sclerosis, apoptosis and bone metastasis in prostate cancer, mitochondrial function and metabolism in ischemic-reperfused hearts, and endoplasmic reticulum neuroreceptor biogenesis.
| Species | Application | Title |
|---|---|---|
ACS Appl Mater Interfaces 3D Artificial Skin Model As a Novel Strategy for the Detection of Pyroptosis-Cascade Activation in Amyotrophic Lateral Sclerosis. | ||
iScience SERPINH1 modulates apoptosis by inhibiting P62 ubiquitination degradation to promote bone metastasis of prostate cancer
| ||
J Proteome Res Data-Independent Acquisition Proteomics and N-Terminomics Methods Reveal Alterations in Mitochondrial Function and Metabolism in Ischemic-Reperfused Hearts | ||
Elife Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum |
Reviews
What customers say
Both reviewers gave this antibody a 5-star rating. One user found it highly effective for binding and blocking HSP47 in human isolated platelets, using it for immunohistochemistry and flow cytometry. The other reported successful Western blot results in mesenchymal stem cells at a 1:2000 dilution in 5% milk. Overall, users report reliable performance across multiple applications with no notable issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Donaldo (Verified Customer) (06-23-2025) | The antibody was very useful to bind/block HSP47 in human isolated platelets.
|
Udesh (Verified Customer) (03-05-2024) | Worked well in WB in MSCs at 1:2000 in 5% milk
|
