Tested Applications
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human prostate cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
| Positive FC (Intra) detected in | Jurkat cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:4000-1:16000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67927-1-Ig targets CREB1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2852 Product name: Recombinant human CREB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC010636 Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Predict reactive species |
| Full Name | cAMP responsive element binding protein 1 |
| Calculated Molecular Weight | 341 aa, 35 kDa |
| Observed Molecular Weight | 43-46 kDa |
| GenBank Accession Number | BC010636 |
| Gene Symbol | CREB1 |
| Gene ID (NCBI) | 1385 |
| RRID | AB_2918679 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P16220 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CREB1 antibody 67927-1-Ig | Download protocol |
| IHC protocol for CREB1 antibody 67927-1-Ig | Download protocol |
| WB protocol for CREB1 antibody 67927-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
67927-1-Ig is a CREB1 antibody with 38 total citations. Citations appear in journals including Nature Communications, EMBO Journal, Journal of Experimental & Clinical Cancer Research, Cell Death & Disease, Phytomedicine, Acta Biomaterialia, Free Radical Biology & Medicine, Aging Cell, ACS Chemical Neuroscience, and others. Research fields span neuroscience (Parkinson's, depression, brain injury), oncology (ferroptosis, cancer stemness, glioblastoma), pharmacology (natural compounds, traditional Chinese medicine), cell signaling (cAMP/PKA/CREB pathway), immunology, and metabolic diseases.
| Species | Application | Title |
|---|---|---|
J Adv Res Targeting FBXO48 with BC1618 promotes axon regeneration and alleviates symptoms of Parkinson's disease. | ||
J Exp Clin Cancer Res A novel lncRNA-mediated signaling axis governs cancer stemness and splicing reprogramming in hepatocellular carcinoma with therapeutic potential. | ||
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Phytomedicine Chinese medicine Sheng Jing Decoction alleviates oligoasthenospermia by modulating PI3K/Akt and MAPK signaling pathways. | ||
Phytomedicine Sweroside, the effective component of jianwei shoutai pills, alleviates recurrent pregnancy loss by activating cAMP signaling pathway in maternal-fetal interface. |
