CREB1 Monoclonal antibody, PBS Only

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  WB analysis using 67927-1-Ig (same clone as 67927-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67927-1-Ig (CREB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

  WB result of CREB1 antibody (67927-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67927-1-Ig (same clone as 67927-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CREB1 antibody (67927-1-Ig, Clone: 1E11C1 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67927-1-Ig (same clone as 67927-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.2 μg CREB1 Monoclonal antibody (67927-1-Ig, Clone:1E11C1,red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005),Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug CREB1 Monoclonal antibody (67927-1-Ig, Clone:1E11C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CREB1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67927-1-PBS

Clone No.1E11C1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1E11C1, cAMP-responsive element-binding protein 1, CREB, CREB 1, CREB-1

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Product Information

67927-1-PBS targets CREB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2852

  Product name: Recombinant human CREB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-341 aa of BC010636

  Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD

• Full Name: cAMP responsive element binding protein 1
• Calculated Molecular Weight: 341 aa, 35 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC010636
• Gene Symbol: CREB1
• Gene ID (NCBI): 1385
• RRID: AB_2918679
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P16220
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).