Tested Applications
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human appendicitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:550-1:2200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68033-1-Ig targets CD35 in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30268 Product name: Recombinant human CR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1740-1971 aa of NM_000573 Sequence: DEGFRLKGRSASHCVLAGMKALWNSSVPVCEQIFCPNPPAILNGRHTGTPFGDIPYGKEISYACDTHPDRGMTFNLIGESSIRCTSDPQGNGVWSSPAPRCELSVPAACPHPPKIQNGHYIGGHVSLYLPGMTISYICDPGYLLVGKGFIFCTDQGIWSQLDHYCKEVNCSFPLFMNGISKELEMKKVYHYGDYVTLKCEDGYTLEGSPWSQCQADDRWDPPLAKCTSRTHD Predict reactive species |
| Full Name | complement component (3b/4b) receptor 1 (Knops blood group) |
| Calculated Molecular Weight | 224 kDa |
| GenBank Accession Number | NM_000573 |
| Gene Symbol | CR1 |
| Gene ID (NCBI) | 1378 |
| ENSEMBL Gene ID | ENSG00000203710 |
| RRID | AB_2918775 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P17927 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD35 antibody 68033-1-Ig | Download protocol |
| IHC protocol for CD35 antibody 68033-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD35 Monoclonal antibody (1B8E6), catalog number 68033-1-Ig, has 1 indexed citation. It was published in Nature Communications (Impact Factor 18.1) in August 2025. The study investigated lymph node molecular subtypes and their heterogeneity with clinical implications in colorectal cancer, using the antibody for immunohistochemistry (IHC).
| Species | Application | Title |
|---|---|---|
Nat Commun Lymph nodes molecular subtypes unravel lymph nodes heterogeneity and clinical implications in colorectal cancer |
