Tested Applications
| Positive WB detected in | NCI-H1299 cells, HeLa cells, NIH/3T3 cells, A549 cells, U2OS cells, A431 cells |
| Positive IF/ICC detected in | DU 145 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68108-1-Ig targets AXL in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14401 Product name: Recombinant human AXL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 545-894 aa of BC032229 Sequence: GEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRRGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA Predict reactive species |
| Full Name | AXL receptor tyrosine kinase |
| Calculated Molecular Weight | 894 aa, 98 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | BC032229 |
| Gene Symbol | AXL |
| Gene ID (NCBI) | 558 |
| RRID | AB_2923637 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P30530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AXL antibody 68108-1-Ig | Download protocol |
| WB protocol for AXL antibody 68108-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review rates the product 3 out of 5 stars. The reviewer reports it works but required optimization. When detecting the target with an anti-mouse 488 secondary antibody, they encountered considerable background and resolved it by increasing BSA percentages in both the blocking and antibody incubation steps. The product was used for immunohistochemistry and immunofluorescence at a 1:100 dilution. Overall, the feedback suggests the antibody is functional but may need protocol adjustments to minimize background in certain applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Asimenia (Verified Customer) (02-16-2026) | Works ok, but required some optimization. I detected it with an Anti-mouse 488 and had quite some background, so I used higher BSA percentages for blocking and antibody incubation.
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